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What (who) is KAHA Ligation - definition
KAHA Ligation
The α-Ketoacid-Hydroxylamine (KAHA) Amide-Forming Ligation is a chemical reaction that is used to join two unprotected fragments in peptide synthesis. It is an alternative to the Native Chemical Ligation (NCL).
Ligation (molecular biology)
![sticky end]] ligation](https://commons.wikimedia.org/wiki/Special:FilePath/Ligation.svg?width=200 "sticky end]] ligation")
TECHNIQUE FOR JOINING DNA MOLECULES IN MOLECULAR BIOLOGY
Blunt-end ligation; DNA ligation
In molecular biology, ligation is the joining of two nucleic acid fragments through the action of an enzyme. It is an essential laboratory procedure in the molecular cloning of DNA, whereby DNA fragments are joined together to create recombinant DNA molecules (such as when a foreign DNA fragment is inserted into a plasmid).
Proximity ligation assay
BIOCHEMICAL METHOD TO DETECT PROTEIN INTERACTIONS
Proximity ligation assay (PLA); Proximity Ligation; RISH-PLA
Proximity ligation assay (in situ PLA) is a technology that extends the capabilities of traditional immunoassays to include direct detection of proteins, protein interactions, Extracellular vesicles and post translational modifications with high specificity and sensitivity. Protein targets can be readily detected and localized with single molecule resolution and objectively quantified in unmodified cells and tissues.
